Search results for "Circulating DNA"

showing 3 items of 3 documents

Direct quantification of cell-free, circulating DNA from unpurified plasma.

2014

Cell-free DNA (cfDNA) in body tissues or fluids is extensively investigated in clinical medicine and other research fields. In this article we provide a direct quantitative real-time PCR (qPCR) as a sensitive tool for the measurement of cfDNA from plasma without previous DNA extraction, which is known to be accompanied by a reduction of DNA yield. The primer sets were designed to amplify a 90 and 222 bp multi-locus L1PA2 sequence. In the first module, cfDNA concentrations in unpurified plasma were compared to cfDNA concentrations in the eluate and the flow-through of the QIAamp DNA Blood Mini Kit and in the eluate of a phenol-chloroform isoamyl (PCI) based DNA extraction, to elucidate the D…

Gene Identification and Analysislcsh:MedicineCoronary DiseaseReal-Time Polymerase Chain ReactionBiochemistrylaw.inventionMolecular Geneticschemistry.chemical_compoundDiagnostic MedicinelawNucleic AcidsMolecular Cell BiologyBlood plasmaGeneticsHumanslcsh:ScienceExerciseBiologyPolymerase chain reactionDNA PrimersPlasma ProteinsMultidisciplinaryBase SequenceCell-Free SystemChemistrylcsh:RProteinsDNAMolecular biologyDNA extractionCoronary heart diseaseReal-time polymerase chain reactionCase-Control StudiesRNAMedicineCirculating DNAlcsh:QGene expressionGene FunctionPrimer (molecular biology)DNA modificationDNAResearch ArticlePLoS ONE
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Kinetics and Topology of DNA Associated with Circulating Extracellular Vesicles Released during Exercise.

2021

AbstractAlthough it is widely accepted that cancer derived extracellular vesicles (EVs) carry DNA cargo, the association of cell-free circulating DNA (cfDNA) and EVs in plasma of healthy humans remains elusive. Using a physiological exercise model, where EVs and cfDNA are synchronously released, we aimed to characterize the kinetics and localization of DNA associated with EVs. EVs were separated from human plasma using size exclusion chromatography or immuno-affinity capture for CD9+, CD63+, and CD81+ EVs. DNA was quantified with an ultra-sensitive qPCR assay targeting repetitive LINE elements, with or without DNase digestion. This model shows that a minute part of circulating cell-free DNA…

AdultMalelcsh:QH426-470KineticsexosomesExtracellular vesiclesPolymerase Chain ReactionArticle796 Athletic and outdoor sports and games570 Life sciencescell-free DNAchemistry.chemical_compoundExtracellular VesiclesYoung Adultphysical exerciseHumansExerciseCD63human plasma796 SportChemistryHealthy VolunteersCell biologylcsh:GeneticsKineticsLong Interspersed Nucleotide ElementsHuman plasmaChromatography GelCirculating DNAFemalecoronavesicular genomic DNACell-Free Nucleic AcidsDNACD81extracellular DNAintraluminal570 BiowissenschaftenGenes
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2018

Background Attempts to establish a biomarker reflecting individual player load in intermittent sports such as football have failed so far. Increases in circulating DNA (cfDNA) have been demonstrated in various endurance sports settings. While it has been proposed that cfDNA could be a suitable marker for player load in intermittent sports, the effects on cfDNA of repeated sprinting as an essential feature in intermittent sports are unknown. For the first time, we assessed both alterations of cfDNA due to repeated maximal sprints and due to a professional football game. Methods Nine participants were subjected to a standardised sprint training session with cross-over design of five maximal s…

0301 basic medicinemedicine.medical_specialtyFootball playersMultidisciplinarybusiness.industry030229 sport sciencesCirculating Cell-Free DNAIncremental exerciseSprint training03 medical and health sciences030104 developmental biology0302 clinical medicineSprintInternal medicinemedicineCardiologyCirculating DNATracking databusinesshuman activitiesPLOS ONE
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